Heterokaryosis and you may parasexual recombination for the pathogenic challenges of Fusarium oxysponrm
V. Heterokaryosis and you will parasexuality
Make use of the “0”spot for one of the biological parents and you will note the stress count with the dish. Use the layout with the replicator. Incubate dos-3 days. Simulate the fresh new segregants for the a series of decide to try plates using an excellent replicator which have, e.g., 21 needles. Draw the brand new plates that have lots. Incubate 2-3 days. Get the exam plates and you can record the fresh phenotypes throughout the scoring dining table. Just be sure to influence the ploidy of your own territories towards the foundation regarding brand new indicators. Look at the ploidy off unsure territories. Create a list of brand new genotypes (you should use a software application). Influence the part of the fresh recombinants into the other indicators. And this indicators was linked? Is it possible you discover intrachromosomal recombination? Where linkage class is the unknown marker?
Within experiment i determine the new gene acquisition and you will venue from the newest centromere for the linkage class VI ofA. niger.Individuals tips for your choice of mitotic recombinants are utilized. The fresh new markers in it try: pubA1, pyrB4, c d l . The fresh new c d locus try critical for the chromosome arm and you may for this reason really appropriate since the possibilities marker. Just like the all markers try recessive, they must be within the cis standing. The chlorate-unwilling segregants will be separated, plus they getting examined on the other indicators. The fresh diploid put was: N761 N640
The new diploid on the MM, cuatro plates CMCIO3 A suspension regarding conidiospores out-of a good diploid nest 3 dishes CM + C103, bottle having saline otherwise sterile liquid 3 dishes CM